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tracy young pearse  (Addgene inc)


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    Structured Review

    Addgene inc tracy young pearse
    Tracy Young Pearse, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tracy+young+pearse/pCAX+APP+Swe%2FInd+(Plasmid+%2330145)/pmc12285154-57-20-22
    Average 93 stars, based on 39 article reviews
    tracy young pearse - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: APP Regulates Microglial Phenotype in a Mouse Model of Alzheimer's Disease
    Article Snippet: A mixture of three N-terminal APP antibodies obtained from EMD Millipore (22C11 clone and N-terminus anti-APP rabbit polyclonal antibody) and Abcam (rabbit polyclonal antibody to APP) was used for the binding assay. .. The human APP plasmid (pCAX APP 695) was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid #30137; Young-Pearse et al., 2007) and the pCMV-RFP plasmid was a gift from Dr. Saobo Lei and Nicholas Cilz. ..

    Article Title: Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway
    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM), PenicillinStreptomycin and culture dishes were from Corning Life Sciences (Tewksbury, MA); Fetal bovine serum (Premium Select) was purchased from Atlanta Biologicals (Flowery Branch, GA); DC Protein Assay Kit, Pre-stained Dual Color Protein Standards, Clarity Western ECL, Chemidoc MP System, Image Lab Software, Chemi Hi Sensitivity blot application, Immuno-Blot LF PVDF membrane, 4–20% Mini Protean TGX Stain Free precast gels, and 4–15% Mini Protean TGX Stain Free precast gels were obtained from BioRad (Hercules, CA); polyvinylidene fluoride (PVDF) transfer membrane (0.45 mm) was purchased from Perkin Elmer (Waltham, MA); Whatman nitro-cellulose transfer membrane (0.2 m) was purchased from GE Healthcare Life Sciences; Lipofectamine 2000, Pierce ECL Western Blotting, Pierce SuperSignal West Pico substrates and G418 sulfate (Geneticin) were all from ThermoFisher Scientific (Waltham, MA); Recombinant Human BACE1 protein, CF (931-AS) and Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate (ES004) were acquired from R&D Systems (Minneapolis, MN); the BACE1 inhibitor LY2811376 was purchased from Selleckchem (Houston, TX). .. pCAX-A PP695 expressing full-length human A PP695 was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid # 30137). pCMV6XL5-BACE1 expressing full-length human BACE1, transcript variant a, NM 012104.3 (# SC115547) was purchased from Origene, Rockville, MD. pSV2neo [33] which provides a selectable marker for resistance to the antibiotic G418 in mammalian cell lines was a gift from David Shalloway, Cornell University. .. Rabbit anti-sA PP (poly8134, 813401) and the mouse monoclonal antibody 6E10 (803001) were purchased from BioLegend (San Diego, CA) and the anti- -tubulin mouse monoclonal antibody (2G7D4, A01717-40) was acquired from GenScript (Piscataway, NJ).

    Article Title: Discoidin Domain Receptor 2 Regulates AT1R Expression in Angiotensin II-Stimulated Cardiac Fibroblasts via Fibronectin-Dependent Integrin-β1 Signaling.
    Article Snippet: The rat DDR2/CD167b Gene ORF cDNA clone expression plasmid was obtained from Sino Biologicals (Beijing, China). .. Pcax Itgb1-FLAG was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid 30153) [46]. .. Opti-MEM and fetal calf serum (FCS) were from GIBCO (Waltham, MA, USA).

    Article Title: New insights into the 17β-hydroxysteroid dehydrogenase type 10 and amyloid-β 42 derived cytotoxicity relevant to Alzheimer's disease.
    Article Snippet: Expi293 Expression Medium (Gibco, A1435101) was used for Western blot analysis, size-exclusion chromatography, and mass-spectrometry analysis of the conditioned culture medium. .. For APPSwe/Ind overexpression, pCAX APP695 Swe/ Ind vector (pCAX APP Swe/Ind was a gift from Dennis Selkoe [21] & Tracy Young-Pearse (Addgene plasmid # 30145; http://n2t.net/addgene:30145; RRID: Addgene_30145)) was used as a template. ..

    Article Title: New insights into the 17β-hydroxysteroid dehydrogenase type 10 and amyloid-β 42 derived cytotoxicity relevant to Alzheimer’s disease
    Article Snippet: Expi293 Expression Medium (Gibco, A1435101) was used for Western blot analysis, size-exclusion chromatography, and mass-spectrometry analysis of the conditioned culture medium. .. For APP Swe/Ind overexpression, pCAX APP695 Swe/Ind vector (pCAX APP Swe/Ind was a gift from Dennis Selkoe [ ] & Tracy Young-Pearse (Addgene plasmid # 30145; http://n2t.net/addgene:30145 ; RRID: Addgene_30145)) was used as a template. ..

    Expressing:

    Article Title: Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway
    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM), PenicillinStreptomycin and culture dishes were from Corning Life Sciences (Tewksbury, MA); Fetal bovine serum (Premium Select) was purchased from Atlanta Biologicals (Flowery Branch, GA); DC Protein Assay Kit, Pre-stained Dual Color Protein Standards, Clarity Western ECL, Chemidoc MP System, Image Lab Software, Chemi Hi Sensitivity blot application, Immuno-Blot LF PVDF membrane, 4–20% Mini Protean TGX Stain Free precast gels, and 4–15% Mini Protean TGX Stain Free precast gels were obtained from BioRad (Hercules, CA); polyvinylidene fluoride (PVDF) transfer membrane (0.45 mm) was purchased from Perkin Elmer (Waltham, MA); Whatman nitro-cellulose transfer membrane (0.2 m) was purchased from GE Healthcare Life Sciences; Lipofectamine 2000, Pierce ECL Western Blotting, Pierce SuperSignal West Pico substrates and G418 sulfate (Geneticin) were all from ThermoFisher Scientific (Waltham, MA); Recombinant Human BACE1 protein, CF (931-AS) and Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate (ES004) were acquired from R&D Systems (Minneapolis, MN); the BACE1 inhibitor LY2811376 was purchased from Selleckchem (Houston, TX). .. pCAX-A PP695 expressing full-length human A PP695 was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid # 30137). pCMV6XL5-BACE1 expressing full-length human BACE1, transcript variant a, NM 012104.3 (# SC115547) was purchased from Origene, Rockville, MD. pSV2neo [33] which provides a selectable marker for resistance to the antibiotic G418 in mammalian cell lines was a gift from David Shalloway, Cornell University. .. Rabbit anti-sA PP (poly8134, 813401) and the mouse monoclonal antibody 6E10 (803001) were purchased from BioLegend (San Diego, CA) and the anti- -tubulin mouse monoclonal antibody (2G7D4, A01717-40) was acquired from GenScript (Piscataway, NJ).

    Variant Assay:

    Article Title: Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway
    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM), PenicillinStreptomycin and culture dishes were from Corning Life Sciences (Tewksbury, MA); Fetal bovine serum (Premium Select) was purchased from Atlanta Biologicals (Flowery Branch, GA); DC Protein Assay Kit, Pre-stained Dual Color Protein Standards, Clarity Western ECL, Chemidoc MP System, Image Lab Software, Chemi Hi Sensitivity blot application, Immuno-Blot LF PVDF membrane, 4–20% Mini Protean TGX Stain Free precast gels, and 4–15% Mini Protean TGX Stain Free precast gels were obtained from BioRad (Hercules, CA); polyvinylidene fluoride (PVDF) transfer membrane (0.45 mm) was purchased from Perkin Elmer (Waltham, MA); Whatman nitro-cellulose transfer membrane (0.2 m) was purchased from GE Healthcare Life Sciences; Lipofectamine 2000, Pierce ECL Western Blotting, Pierce SuperSignal West Pico substrates and G418 sulfate (Geneticin) were all from ThermoFisher Scientific (Waltham, MA); Recombinant Human BACE1 protein, CF (931-AS) and Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate (ES004) were acquired from R&D Systems (Minneapolis, MN); the BACE1 inhibitor LY2811376 was purchased from Selleckchem (Houston, TX). .. pCAX-A PP695 expressing full-length human A PP695 was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid # 30137). pCMV6XL5-BACE1 expressing full-length human BACE1, transcript variant a, NM 012104.3 (# SC115547) was purchased from Origene, Rockville, MD. pSV2neo [33] which provides a selectable marker for resistance to the antibiotic G418 in mammalian cell lines was a gift from David Shalloway, Cornell University. .. Rabbit anti-sA PP (poly8134, 813401) and the mouse monoclonal antibody 6E10 (803001) were purchased from BioLegend (San Diego, CA) and the anti- -tubulin mouse monoclonal antibody (2G7D4, A01717-40) was acquired from GenScript (Piscataway, NJ).

    Marker:

    Article Title: Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway
    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM), PenicillinStreptomycin and culture dishes were from Corning Life Sciences (Tewksbury, MA); Fetal bovine serum (Premium Select) was purchased from Atlanta Biologicals (Flowery Branch, GA); DC Protein Assay Kit, Pre-stained Dual Color Protein Standards, Clarity Western ECL, Chemidoc MP System, Image Lab Software, Chemi Hi Sensitivity blot application, Immuno-Blot LF PVDF membrane, 4–20% Mini Protean TGX Stain Free precast gels, and 4–15% Mini Protean TGX Stain Free precast gels were obtained from BioRad (Hercules, CA); polyvinylidene fluoride (PVDF) transfer membrane (0.45 mm) was purchased from Perkin Elmer (Waltham, MA); Whatman nitro-cellulose transfer membrane (0.2 m) was purchased from GE Healthcare Life Sciences; Lipofectamine 2000, Pierce ECL Western Blotting, Pierce SuperSignal West Pico substrates and G418 sulfate (Geneticin) were all from ThermoFisher Scientific (Waltham, MA); Recombinant Human BACE1 protein, CF (931-AS) and Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate (ES004) were acquired from R&D Systems (Minneapolis, MN); the BACE1 inhibitor LY2811376 was purchased from Selleckchem (Houston, TX). .. pCAX-A PP695 expressing full-length human A PP695 was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid # 30137). pCMV6XL5-BACE1 expressing full-length human BACE1, transcript variant a, NM 012104.3 (# SC115547) was purchased from Origene, Rockville, MD. pSV2neo [33] which provides a selectable marker for resistance to the antibiotic G418 in mammalian cell lines was a gift from David Shalloway, Cornell University. .. Rabbit anti-sA PP (poly8134, 813401) and the mouse monoclonal antibody 6E10 (803001) were purchased from BioLegend (San Diego, CA) and the anti- -tubulin mouse monoclonal antibody (2G7D4, A01717-40) was acquired from GenScript (Piscataway, NJ).

    Over Expression:

    Article Title: New insights into the 17β-hydroxysteroid dehydrogenase type 10 and amyloid-β 42 derived cytotoxicity relevant to Alzheimer's disease.
    Article Snippet: Expi293 Expression Medium (Gibco, A1435101) was used for Western blot analysis, size-exclusion chromatography, and mass-spectrometry analysis of the conditioned culture medium. .. For APPSwe/Ind overexpression, pCAX APP695 Swe/ Ind vector (pCAX APP Swe/Ind was a gift from Dennis Selkoe [21] & Tracy Young-Pearse (Addgene plasmid # 30145; http://n2t.net/addgene:30145; RRID: Addgene_30145)) was used as a template. ..

    Article Title: New insights into the 17β-hydroxysteroid dehydrogenase type 10 and amyloid-β 42 derived cytotoxicity relevant to Alzheimer’s disease
    Article Snippet: Expi293 Expression Medium (Gibco, A1435101) was used for Western blot analysis, size-exclusion chromatography, and mass-spectrometry analysis of the conditioned culture medium. .. For APP Swe/Ind overexpression, pCAX APP695 Swe/Ind vector (pCAX APP Swe/Ind was a gift from Dennis Selkoe [ ] & Tracy Young-Pearse (Addgene plasmid # 30145; http://n2t.net/addgene:30145 ; RRID: Addgene_30145)) was used as a template. ..



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    Addgene inc tracy young pearse
    Tracy Young Pearse, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tracy+young+pearse/pCAX+APP+Swe%2FInd+(Plasmid+%2330145)/pmc12285154-57-20-22
    Average 93 stars, based on 1 article reviews
    tracy young pearse - by Bioz Stars, 2026-09
    93/100 stars
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